Terenzio, M. et al. Sci. Kadyk, L. C. & Kimble, J. Presynaptic protein synthesis is required for long-term plasticity of GABA release. Fluorescence microscopy-based technique that allows the visualization and quantification of single mRNAs in fixed prokaryotic or eukaryotic cells. 12, 1351 (2020). Paquin, N. et al. Tian, L. & Okita, T. W. mRNA-based protein targeting to the endoplasmic reticulum and chloroplasts in plant cells. Nature 390, 477484 (1997). 1999).To test whether BCD can associate with these cap-bound proteins, we produced cytoplasmic extracts of early Drosophila embryos and . Mol. Local translation of mRNA provides a mechanism for efficient protein sorting shaping cell function. Song, W. et al. Biol. Stress granules and processing bodies are dynamically linked sites of mRNP remodeling. Recognition of a bicoid mRNA localization signal by a protein complex containing Swallow, Nod, and RNA binding proteins. Identification of cis-localization elements that target glutelin RNAs to a specific subdomain of the cortical endoplasmic reticulum in rice endosperm cells. Kim, N. Y. et al. Synonymous modification results in high-fidelity gene expression of repetitive protein and nucleotide sequences. Binding of a phosphoprotein to the 3 untranslated region of the mouse protamine 2 mRNA temporally represses its translation. This study demonstrates co-transport of mRNAs with lysosomes in axons, with implications in amyotrophic lateral sclerosis. 219, e202004120 (2020). Natl Acad. The authors declare no competing interests. The first study to identify the local translatome in the developing and mature nervous system. Mol. Chicurel, M. E., Singer, R. H., Meyer, C. J. A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting. Tool for the identification of proteinprotein interactions based on the in vivo labelling with biotin of interactors located in close proximity to the candidate protein. Morales, C. R. et al. Hubstenberger, A. et al. Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control. Am. Aronov, S. et al. Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, New York, NY, USA, Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, NY, USA, Department of Biochemistry, McGill University, Montreal, Quebec, Canada, Janelia Research Campus of the HHMI, Ashburn, VA, USA, Systems Biology Lab, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit Amsterdam, Amsterdam, The Netherlands, You can also search for this author in Genes Dev. Dev. bicoid RNA localization requires specific binding of an endosomal Bicoid is a homeodomain transcription factor that not only regulates the transcription of segmentation genes, but also controls the translation of the caudal mRNA that is also involved in axis . Clark, I. E., Wyckoff, D. & Gavis, E. R. Synthesis of the posterior determinant Nanos is spatially restricted by a novel cotranslational regulatory mechanism. Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. & Singer, R. H. Intracellular localization of messenger RNAs for cytoskeletal proteins. PLoS Genet. Biol. Development 126, 49894996 (1999). Biochem. Mitochondria-associated yeast mRNAs and the biogenesis of molecular complexes. RNA world (article) | Natural selection | Khan Academy & Parker, R. mRNP architecture in translating and stress conditions reveals an ordered pathway of mRNP compaction. 28, 18551866 (2009). Mol. Cell 84, 687697 (1996). 125, 27402752 (2012). We also thank T. Schedl for allowing us to discuss unpublished results. Safieddine, A. et al. Biol. J. Nature 438, 512515 (2005). Richter, J. D. & Sonenberg, N. Regulation of cap-dependent translation by eIF4E inhibitory proteins. Expression of messenger RNA ( mRNA) transcripts is controlled in time and space so that the protein products of these transcripts appear in gradients along each fertilized egg. 10, 14791488 (2000). 35, 365406 (2001). Article Martin, K. C. et al. RNA 22, 660666 (2016). RNA https://doi.org/10.1002/wrna.1453 (2018). 4, eaar3448 (2018). Hocine, S., Raymond, P., Zenklusen, D., Chao, J. Brodsky, A. S. & Silver, P. A. Identifying proteins that affect mRNA localization in living cells. The experiments show that another motor protein called Dynein moves bicoid mRNA along microtubules. volume22,pages 483504 (2021)Cite this article, An Author Correction to this article was published on 28 April 2021. Front. A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germline. Sonoda, J. EMBO J. Sci. Proc. High efficiency of HIV-1 genomic RNA packaging and heterozygote formation revealed by single virion analysis. To obtain Proc.Natl Acad. Ogura, K., Kishimoto, N., Mitani, S., Gengyo-Ando, K. & Kohara, Y. Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein SPN-4 in Caenorhabditis elegans. Seydoux, G. The P granules of C. elegans: a genetic model for the study of RNAprotein condensates. Nat. Cell 4, 625637 (2003). Res. Rev. Biol. Eliscovich, C., Shenoy, S. M. & Singer, R. H. Imaging mRNA and protein interactions within neurons. nanoCAGE reveals 5 UTR features that define specific modes of translation of functionally related MTOR-sensitive mRNAs. Yang, L.-Z. Kindler, S., Wang, H., Richter, D. & Tiedge, H. RNA transport and local control of translation. The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo. 27, 34413455 (2007). -Actin mRNA interactome mapping by proximity biotinylation. Mol. Article Mol. How introns influence and enhance eukaryotic gene expression. 15, R4 (2014). Proc. Annu. Stress-specific differences in assembly and composition of stress granules and related foci. Finally, we summarize the technological developments that allow us to study mRNA localization and local translation through the simultaneous detection of mRNAs and proteins in single cells, mRNA and nascent protein single-molecule imaging, and bulk RNA and protein detection methods. Multicolour single-molecule tracking of mRNA interactions with RNP granules. & Simonelig, M. Oskar allows nanos mRNA translation in Drosophila embryos by preventing its deadenylation by Smaug/CCR4. Nat. Giuditta, A., Cupellot, A. Cell Biol. 19, 236243 (2009). Methods 26, 151155 (2002). Article 9, 337340 (1999). Sci. Voigt, F. et al. Cell 42, 769777 (1985). Atlas of subcellular RNA localization revealed by APEX-Seq. Subramanian, M. et al. Traffic 13, 16421652 (2012). Proc. USA 106, 1353513540 (2009). Kennerdell, J. R., Yamaguchi, S. & Carthew, R. W. RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E. 16, 15301536 (2013). Curr. 202, 10411055 (2013). Cell 75, 843854 (1993). Genes Cancer 4, 369377 (2013). Rev. Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation. Struct. Genes Dev. J. Neurosci. An enzyme that separates the two nucleic acid strands in a double helix, which results in the formation of regions of single-stranded DNA or RNA. Localized translation near the mitochondrial outer membrane: an update. Many crucial decisions, such as the location and timing of cell division, cell-fate determination, and embryonic axes establishment, are made in the early embryo, a time in development when there is often little or no transcription. Cap and cap-binding proteins in the control of gene expression. McBride, A. E. Messenger RNA transport in the opportunistic fungal pathogen Candida albicans. Cell 6, 12531259 (2000). Costantini, L. M., Fossati, M., Francolini, M. & Snapp, E. L. Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions. Cell 181, 325345.e28 (2020). 8, 896 (2017). S. et al. 204, 343357 (2014). 63, 989995 (2017). Biol. Smibert, C. A., Wilson, J. E., Kerr, K. & Macdonald, P. M. Smaug protein represses translation of unlocalized nanos mRNA in the Drosophila embryo. Markussen, F. H., Breitwieser, W. & Ephrussi, A. Specific interaction of KIF11 with ZBP1 regulates the transport of beta-actin mRNA and cell motility. Wang, C., Dickinson, L. K. & Lehmann, R. Genetics of nanos localization in Drosophila. Francis, R., Barton, M. K., Kimble, J. Sci. 131, jcs196808 (2018). Int. & Sharma, C. M. RNA localization in bacteria. Microbiol. Signal amplification system for the visualization of proteins in living or fixed cells. 20, 285302 (2019). Shigeoka, T. et al. Cell 178, 458472.e19 (2019). RNA 11, e1587 (2020). Proc. 75, 17541762 (2000). Neuron 92, 479492 (2016). Bakshi, S., Choi, H. & Weisshaar, J. C. The spatial biology of transcription and translation in rapidly growing Escherichia coli. Biol. Mol. Natl Acad. 29, 534548 (2019). The proper spatial expression of downstream genes relies on the robustness of this gradient to common variations between embryos, including in the number of maternally-deposited bicoid mRNAs and in egg size. Neuron 95, 852868.e8 (2017). Genome Biol. & Bartel, D. P. MicroRNAs in plants. Biol. Developmental Biology 3230 - University of Utah Puromycin reactivity does not accurately localize translation at the subcellular level. Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, 53706, Wisconsin, USA, You can also search for this author in & Jansen, R.-P. Localization of a subset of yeast mRNAs depends on inheritance of endoplasmic reticulum. Article CAS Sci. & Wharton, R. P. Binding of Pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos. RNA 17, 20492057 (2011). Femino, A. M., Fay, F. S., Fogarty, K. & Singer, R. H. Visualization of single RNA transcripts in situ. Late endosomes act as mRNA translation platforms and sustain mitochondria in axons. Google Scholar. 2, 521529 (2001). Murata, Y. Ostroff, L. E., Fiala, J. C., Allwardt, B. Google Scholar. Elson, S. L., Noble, S. M., Solis, N. V., Filler, S. G. & Johnson, A. D. An RNA transport system in Candida albicans regulates hyphal morphology and invasive growth. Gamberi, C., Peterson, D. S., He, L. & Gottlieb, E. An anterior function for the Drosophila posterior determinant Pumilio. Fungal Biol. Biol. Wu, B. et al. Natl Acad. Cell 87, 205216 (1996). Neuron 60, 201214 (2008). Some Bicoid protein is likely also translated off of the lower concentration Bicoid mRNA that has moved posteriorly via microtubules. Nat. Padrn, A., Iwasaki, S. & Ingolia, N. T. Proximity RNA labeling by APEX-Seq reveals the organization of translation initiation complexes and repressive RNA granules. Proc. RNA docking and local translation regulate site-specific axon remodeling in vivo. Lee, R. C., Feinbaum, R. L. & Ambros, V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Moffitt, J. R., Pandey, S., Boettiger, A. N., Wang, S. & Zhuang, X. Spatial organization shapes the turnover of a bacterial transcriptome. Garcia, J. F. & Parker, R. MS2 coat proteins bound to yeast mRNAs block 5 to 3 degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system. Methods 5, 877879 (2008). Nat. Dev. Sci. The yeast nuclear cap binding complex can interact with translation factor eIF4G and mediate translation initiation. It signals to the germline to maintain mitotic proliferation. Ho, J. J. D. & Lee, S. A cap for every occasion: alternative eIF4F complexes. RNA 20, 429439 (2014). PubMed Knowles, R. B. et al. 15, 284294 (2005). Bartlett, W. P. & Banker, G. A. Micklem, D. R., Adams, J., Grunert, S. & St. Johnston, D. Distinct roles of two conserved Staufen domains in oskar mRNA localization and translation. Traffic 21, 404418 (2020). Asymmetric division of P1, until P4 leads to the production of the germline tissue. PubMed Khong, A. EMBO J. Mendez, R., Murthy, K. G., Ryan, K., Manley, J. L. & Richter, J. D. Phosphorylation of CPEB by Eg2 mediates the recruitment of CPSF into an active cytoplasmic polyadenylation complex. Many crucial decisions made during development are regulated by elements that are located in the 3 untranslated region (3 UTR) that control translation. Plant Cell 15, 22652272 (2003). Eur. Sci. 31, 553562 (2006). Mechanisms that have been proposed to effect this scaling include non-linear degradation of Bicoid,[19] nuclear retention as a size-dependent regulator of Bicoid protein's effective diffusion coefficient,[10][20] and scaling of cytoplasmic streaming.[10]. PubMed Opin. Lionnet, T. et al. Homeotic protein bicoid is encoded by the bcd maternal effect gene in Drosophilia. Science 298, 19461950 (2002). Garcia-Jove Navarro, M. et al. Shiber, A. et al. Chouaib, R. et al. mRNA localization: an orchestration of assembly, traffic and synthesis 16, 15381543 (2006). Commun. Moissoglu, K., Yasuda, K., Wang, T., Chrisafis, G. & Mili, S. Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport. Optogenetic control of mRNA localization and translation in live cells. 3, 373382 (2008). Abstract We show that mRNA diffusion is the main morphogenesis mechanism that consistently explains the establishment of Bicoid protein gradients in the embryo of Drosophila, contradicting the current view of protein diffusion. & Hentze, M. W. Translational control of dosage compensation in Drosophila by Sex-lethal: cooperative silencing via the 5 and 3 UTRs of msl-2 mRNA is independent of the poly(A) tail. Developmental regulation of bicoid mRNA stability is mediated by the Biol. Trends Biol. Traffic 9, 12561267 (2008). Different protein concentration thresholds activate different gap genes along the anterior half of the embryo (11, 12). PLoS ONE 3, e2293 (2008). This paper demonstrates the intrinsic ability of mRNAs to self-organize as homotypic assemblies within Drosophila germ granules. Giorgini, F., Davies, H. G. & Braun, R. E. MSY2 and MSY4 bind a conserved sequence in the 3 untranslated region of protamine 1 mRNA in vitro and in vivo. Edelmann, F. T. et al. [9] Translation of bicoid is regulated by its 3 UTR and begins after egg deposition. Development 121, 37233732 (1995). 22, 45354543 (2002). Safieddine, A. et al. Gomes, J. E. et al. Mol. Nat. mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3 end. Cell 72, 727738.e5 (2018). Vourekas, A., Alexiou, P., Vrettos, N., Maragkakis, M. & Mourelatos, Z. Sequence-dependent but not sequence-specific piRNA adhesion traps mRNAs to the germ plasm. 27, 891903 (2020). Natl Acad. Regulation of ribosomal recruitment in eukaryotes (Cold Spring Harbor Press, Cold Spring Harbor, New York, 2000). 213, 120126 (2003). Tutucci, E. et al. Curr. Mardakheh, F. K. et al. Tyagi, S. & Kramer, F. R. Molecular beacons: probes that fluoresce upon hybridization. A stemloop structure directs oskar mRNA to microtubule minus ends. PubMed Open Access First demonstration of single mRNA detection in situ by FISH. Proc. Wickens, M., Goodwin, E. B., Kimble, J., Strickland, S. & Hentze, M. W. in Translational Control of Gene Expression (eds. Sci. The structural basis for RNA selectivity by the IMP family of RNA-binding proteins. Kim-Ha, J., Kerr, K. & Macdonald, P. M. Translational regulation of oskar mRNA by Bruno, an ovarian RNA-binding protein, is essential. 18, 61466154 (1999). Natl Acad. Google Scholar. Cell Rep. 4, 11441155 (2013). Cajigas, I. J. et al. 22, 341352 (2020). Programmable RNA tracking in live cells with CRISPR/Cas9. 18, 572577 (2002). Davies, H. G., Giorgini, F., Fajardo, M. A. Cell Biol. 10, 3230 (2019). smFISH coupled with super-resolved imaging is used to study the mRNA conformation within the mRNP as a function of the translation state. Gu, W., Deng, Y., Zenklusen, D. & Singer, R. H. A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization. Huang, N. N., Mootz, D. E., Walhout, A. J., Vidal, M. & Hunter, C. P. MEX-3 interacting proteins link cell polarity to asymmetric gene expression in Caenorhabditis elegans. & Hecht, N. B. Reference 113 shows how Maskin silences mRNA translation by binding to and sequestering eIF4E during oocyte maturation. 17, 21582165 (1997). 18, 14521465 (2004). Struct. https://europepmc.org/article/med/10635326 (2001). Drosophila embryogenesis - Wikipedia Garcia, H. & Gregor, T. Live imaging of mRNA synthesis in Drosophila. Moor, A. E. et al. The stem loop structures of bicoid mRNA recruit the double-strand RNA binding protein, Staufen, which has been demonstrated to be important for the posterior localization of bicoid mRNA . Tian, L., Chou, H.-L., Fukuda, M., Kumamaru, T. & Okita, T. W. mRNA localization in plant cells. Egg-polarity Egg-polarity genes are responsible for establishing the polarity of the egg. Sepulveda, G. et al. FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the C. elegans hermaphrodite germline. Cell 5, 671682 (2000). Methods 15, 8189 (2018). E.B.G. Development 125, 14871494 (1998). Development 124, 24632476 (1997). ISSN 1471-0072 (print). Intracellular mRNA transport and localized translation. Curr. Draper, B. W., Mello, C. C., Bowerman, B., Hardin, J. Genes Dev. Brennecke, J., Hipfner, D. R., Stark, A., Russell, R. B. EMBO J. J. This technique allowed the detection of RNAs with nanoscale precision in mammalian tissue by de-crowding of mRNAs and amplification of single-molecule signals. Rev. Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins. Mol. Sci. Metrics bicoid messenger RNA localizes to the anterior of the Drosophila egg, where it is translated to form a morphogen gradient of Bicoid protein that patterns the head and thorax of the. Curr. Nat. Reference 35 shows that MEX-5, MEX-6 and SPN-4, which function downstream of PAR-1, a protein central to the establishment of embryonic polarity, link cell polarity with asymmetric gene expression by regulating the activity of MEX-3, a translational repressor of pal-1. mTORC1 controls mitochondrial activity and biogenesis through 4E-BP-dependent translational regulation. eLife 8, e48718 (2019). In several organisms, including Drosophila and Caenorhabditis elegans, cascades of translational regulators have central roles in tissue patterning and embryonic axis formation. Soc. Coordination of endoplasmic reticulum and mRNA localization to the yeast bud. The RNA-binding protein, TB-RBP, is the mouse homologue of translin, a recombination protein associated with chromosomal translocations. The maternal gene spn-4 encodes a predicted RRM protein required for mitotic spindle orientation and cell fate patterning in early C. elegans embryos. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in The Drosophila splicing regulator sex-lethal directly inhibits translation of male-specific-lethal 2 mRNA. Genes Dev. 10, 26002609 (1996). Topisirovic, I., Svitkin, Y. V., Sonenberg, N. & Shatkin, A. J. Reference 85 reports the cloning and characterization of the lin-4 mRNA, the first miRNA to be cloned. Biever, A. et al. Nature 419, 312316 (2002). Reprod. Gray, N. K. & Wickens, M. Control of translation initiation in animals. Cioni, J.-M. et al. Wu, B., Eliscovich, C., Yoon, Y. J. Becker, T., Song, J. Woodruff, J. Open Access articles citing this article. First use of the MS2 system for real-time imaging of single mRNAs in live cells. Younts, T. J. et al. Le Hir, H., Nott, A. Cell 172, 590604.e13 (2018). 118, 24252433 (2005). Opin. 1649, 349357 (2018). Neuron 51, 685690 (2006). Rudel, D. & Kimble, J. 17, 21072121 (1998). 13, 22682296 (2018). & Vale, R. D. The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p. Cell 80, 747756 (1995). Translocation of RNA granules in living neurons. Rev. Cell Biol. Dev. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. & Vale, R. D. Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast. Biol. Natl Acad. Transcription Factor Cascades and Segmentation | Learn Science - Nature Mendez, R. et al. Trends Cell Biol. Moss, E. G. & Poethig, R. S. MicroRNAs: something new under the sun. Cell 178, 473490.e26 (2019). Signalling molecules, the distribution pattern of which within an organism contributes to cell differentiation, the determination of anteriorposterior body axis and development. PubMed Parker, D. M. et al. Cell. & Hirokawa, N. Components of RNA granules affect their localization and dynamics in neuronal dendrites. CAS Cell 16, 58195831 (2005). 57, 110116 (2019). Annu. 15, 103105 (2008). 26, 557566 (2019). Cell 183, 18011812.e13 (2020). Curr. Google Scholar. Follow the mRNA: a new model for Bicoid gradient formation & Anzai, K. The dendritic translocation of translin protein in the form of BC1 RNA protein particles in developing rat hippocampal neurons in primary culture. Science 343, 419422 (2014). Biochim. Nature Rev. & Moore, M. J. Locally translated mTOR controls axonal local translation in nerve injury. Chapter 21 Flashcards | Quizlet Cell Sci. P-body purification reveals the condensation of repressed mRNA regulons. 2166, 121144 (2020). Bicoid associates with the 5-cap-bound complex of caudal mRNA and A motor protein called kinesin-1 transports the oskar mRNA to the rear end, but how bicoid mRNA moves to the front end is not clear. The power of the 3 UTR: translational control and development Park, H. Y., Trcek, T., Wells, A. L., Chao, J. Opin. 04 April 2023, Nature Communications Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates. Lecuyer, E. et al. Microbiol. The Bicoid Stability Factor Controls Polyadenylation and - PLOS Nat. Castellana, M., Hsin-Jung Li, S. & Wingreen, N. S. Spatial organization of bacterial transcription and translation. Yoon, Y. J. et al. RNA 5, 15351547 (1999). Trends Genet. Cell Res. Engel, K. L., Arora, A., Goering, R., Lo, H.-Y. Vincent, T. et al. Treeck, B. V. et al. Article Kwon, O. S. et al. Mol. Washida, H. et al. 295370 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2000). USA 116, 1286312872 (2019). Biol. Cell 169, 10661077.e10 (2017). Global analysis of mRNA, translation, and protein localization: local translation is a key regulator of cell protrusions. Open Access After 3 h, the nuclei at the 14th nuclear cycle are filled with Bcd protein which forms a precise morphogen gradient ( f ). 13, 27042712 (1999). Evidence that let-7 , an miRNA, is highly conserved and that it is processed from a stem-loop precursor. Nat. Xia, C., Fan, J., Emanuel, G., Hao, J. Cell 75, 329339 (1993). Wu, H., Zhou, J., Zhu, T., Cohen, I. Nature 408, 8689 (2000). Gmez, G. & Palls, V. Noncoding RNA mediated traffic of foreign mRNA into chloroplasts reveals a novel signaling mechanism in plants. Science 367, eaay4991 (2020). Cell Sci. Annu. Leung, K.-M. et al. & Schuman, E. M. Differential regulation of local mRNA dynamics and translation following long-term potentiation and depression. At the single-cell level, mRNA trafficking dictates cell polarity, motility and differentiation by enabling rapid and localized responses to intracellular and extracellular signals, yet. Determining the scale of the Bicoid morphogen gradient - PNAS Dahanukar, A., Walker, J. Curr. Curr. and M.V. & Warren, S. T. Fragile X syndrome: loss of local mRNA regulation alters synaptic development and function. Cell 181, 346361.e17 (2020). Development 126, 32633274 (1999). Hobson, B. D., Kong, L., Hartwick, E. W., Gonzalez, R. L. & Sims, P. A. Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes. Sonoda, J. Pagliarini, D. J. et al. 13, 11591168 (2003). 13, 11871194 (2017). A multiple ribosomal structure in protein synthesis. Translation- and SRP-independent mRNA targeting to the endoplasmic reticulum in the yeast Saccharomyces cerevisiae. Development 129, 26992710 (2002). [15] Bicoid contains an arginine-rich motif (part of the helix shown axially in this image) that is similar to the one found in the HIV protein REV and is essential for its nucleic acid binding. Reinhart, B. J., Weinstein, E. G., Rhoades, M. W., Bartel, B. Pasquinelli, A. E. et al. Markussen, F. H., Michon, A. M., Breitwieser, W. & Ephrussi, A. Translational control of oskar generates short OSK, the isoform that induces pole plasma assembly. Sci. Genes Dev. & Tuschl, T. New microRNAs from mouse and human. Commun. Cell 66, 3750 (1991). Cell 76, 981997.e7 (2019). Mol. 62, 1317 (1997). Cell 68, 144157.e5 (2017). Knig, J. et al. RNA Biol. The fungal RNA-binding protein Rrm4 mediates long-distance transport of ubi1 and rho3 mRNAs. & Arava, Y. Science 280, 585590 (1998). Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1. USA 98, 70807085 (2001). Identification of asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene. & Liu, G. An RNA-zipcode-independent mechanism that localizes Dia1 mRNA to the perinuclear ER through interactions between Dia1 nascent peptide and Rho-GTP. Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function.