Genet Mol Biol. The Facility offers services including DNA pronuclear injection into fertilized oocytes (along with genotyping of transgenic founders), ES cell injection into blastocysts, cytoplasmic/pronuclear injections into fertilized oocytes of CRISPR-Cas9 mix (gRNA, Cas9RNA, ssDNA/dsDNA templates), embryo and sperm cryopreservation, in vitro fertilization, and re-derivation of live and cryopreserved lines. Phone: 215-573-2301, 3800 Spruce Street - Philadelphia, PA 19104, Richard King Mellon Professor of Reproductive Physiology, Research Professor of Obstetrics and Gynecology, Assistant Professor, Department of Biomedical Sciences, Associate Professor, CE, of Medicine and Reproduction, Associate Professor, Section of Reproduction and Behavior, New Bolton Center, Clinical Assistant Professor, Biomedical Sciences, School of Veterinary Medicine. 3800 Spruce Street - Philadelphia, PA 19104. What strain are the donor embryos used for microinjection? D. David C. Schultz, Ph. Phone: 215-573-2302, Stephanie Sterling, Lab Manager 1 Through intramolecular recombination, cre has the ability to excise loxP-flanked (floxed) sequences from the genome, leaving a single loxP site in its place. Transgenic and Chimeric Mouse Facility - Penn Genetics The efficiency and extent of mutagenic activity of some new mutagens of base-analogue type. Philadelphia, PA 19104 About | Emory University | Atlanta GA This should result in reduction in effort and cost to participating investigators. For more information about our services, charges for services or personnel please visit the official Transgenic & Chimeric Mouse Facility. These mice carry randomly inserted transgenes and/or site-specific alterations in the mouse genome of specific interest to Penn researchers. 8600 Rockville Pike Transgenic Core. Basic services offered are DNA injections, embryonic stem cell injections, embryo freezing and line rescue. It is the policy of the Core that no injection date will be assigned until the DNA to be injected is submitted, the service request has been completed Online and the required documents have been provided. Mutat Res. All Mice Considered - Genetically Engineered Murine Model (GEMM) Core 8 purine base analogues have been found that induce the AT----CG transversion at the trp A88 site; and 2-amino-6-methylaminopurine (2A6MAP) stimulates by 124-fold, 2-amino-6-ethylaminopurine by 20-fold, 2-methylaminopurine (2MAP) by 9.4-fold, 2,6-bismethylaminopurine by 25-fold, 2AP by 230-fold, 2,6DAP by 15-fold, 2.6-diaminopurine riboside by 5-fold, and 2-hydroxylaminopurine by 11-fold. The mice featured are three littermates generated by the injection of ES cells into mouse blastocysts. The mouse core facility at Boston University School of Medicine provides centralized, expert service to produce successfully transgenic as well as knock . vendor, received from a collaborator, custom-made by a core or created in the lab. Penn Vet | Services - University of Pennsylvania School of Veterinary Would you like email updates of new search results? Study and manipulation of male germ line stem cells has implications for control of fertility, large animal transgenesis and genetic preservation. U Penn. UPenn users. Evidence is presented that hydroxylaminopurine mutagenesis is caused by a covalent reaction of these compounds with DNA. 5/28/2019 (Penn Biosafety) Disposal of Transgenic Arthropods & Transgenic Mouse Core Pricing This image shows the small intestinal epithelium, where epithelial cell membranes are labeled in red. PDF Universiti Putra Malaysia Ecto-and Endo-parasites in Rodents and - Core Department of Genetics Perelman School of Medicine Clinical Research Building . Smilow Center for Translational Research Introduction to Anatomy and Physiology II with Lab or Human Anatomy with Lab. Philadelphia, PA 19104. We do not charge you a service fee unless we produce a founder. D. dschultz@mail.med.upenn.edu (215) 5739641 . Kristen L. Kucharczuk, Cheri M. Love, Nancy M. Dougherty, David J. Goldhamer; Fine-scale transgenic mapping of the MyoD core enhancer: MyoD is regulated by distinct but overlapping mechanisms in myotomal and non . This facility consists of an animal room and several injection rooms. Because transgenic full-length amelogenin partially rescues both density and volume of KO molar enamel, it was concluded that the amelogenin C-terminus is essential for proper enamel density, volume, and organization. Site Design: DART Web Team. He will coordinate ULAR interactions and facility maintenance, and be directly responsible for scientific consultations relevant to facility use. Philadelphia, PA 19104-6376, Introduction to Anatomy and Physiology 1 with Lab or Human Anatomy with Lab, Introduction to Anatomy and Physiology II with Lab or Human Anatomy with Lab, If you have a bachelors degree in a non-nursing discipline or are an RN Return Student (you have completed a diploma RN program or an associates degree RN program), you must apply to the. Accessibility The Transgenic Mouse Coreis equipped with state-of-the-art equipment and is supported by fully trained personnel. Penn Vet | Contact Us Core Director piszczeg@nhlbi.nih.gov DNA Sequencing and Genomics Core The DNA Sequencing and Genomics Core provides NHLBI investigators with fast and cost-effective next-generation sequencing services to facilitate DIR basic and translational research. D. David C. Schultz, Ph. The population aging and an increased life expectancy are widely recognized social changes. The Transgenic Mouse Genome Editing Core has generated a wide range of transgenic and gene-targeted mouse lines that have proven to be powerful tools for the study of the molecular biology and physiology of Type I and Type II diabetes, obesity, and developmental and mechanistic bases for a wide variety of other disorders of endocrinology and met. What do I do with my Chimeras after transfer? We thank Jean Richa and the University of Pennsylvania Transgenic Core Facility and Ray Meade and the University of Pennsylvania Biomedical Imaging Core Facility for electron microscopy analysis and the Jefferson Kimmel Cancer Center Bioimaging Facility for confocal imaging. Stem Cell and Xenograft Core Transgenic and Chimeric Mouse Facility Capitalizing on years of foundation research, the Center for Animal Transgenesis and Germ Cell Research was established in 1998. Mutat Res. It also allows for ease of sharing mouse strains with collaborators across the world. Cores - Penn Genetics Advising@Penn. The Trustees of the University of Pennsylvania. 2010 Jul;33(3):549-57. doi: 10.1590/S1415-47572010005000060. Mutat Res. Study of genetic diseases in domestic animals. Dr. Richa will maintain direct contact with investigators using the facility. What strain of mice are used as foster mothers? Association of Biomolecular Resource Facilities. University of Pennsylvania - ASLAP Photograph by . We offer a 20% discount on your Injections if you choose to utilize our in house Es Cell derivation services. It is located in a barrier, pathogen-free small animal facility supervised by trained veterinarians. Technologies are believed to improve the elderly's daily lives and maintain their health efficiently. The tryptophan synthetase gene A series of mutants in E. coli has been used to examine the mutational specificity of over 80 purine base analogues. They may also result from the crossing of one or more transgenic animals. Care for cats, dogs & other companion animals. These genetically altered organisms, called transgenic, and are of enormous value in medicine and agriculture. 5-Amino-4-imidazolecarboxamide is a mutagen in E. coli. Accounting, Department of (Wharton) Accounts Payable (University) Ackoff Center for Advancement of Systems Approaches (ACASA) Acquisition Services. The Facility offers services including DNA pronuclear injection into fertilized oocytes (along with genotyping of transgenic founders), ES cell injection into blastocysts, cytoplasmic/pronuclear injections into fertilized oocytes of CRISPR-Cas9 mix (gRNA, Cas9RNA, ssDNA/dsDNA templates), embryo and sperm cryopreservation, in vitro fertilization, and re-derivation of live and cryopreserved lines. Associate Dean of Sustainable Agriculture and Veterinary Practices Disclaimer. Major categories . However there is a clear indication that such development is shifting towards 1 Volume 1, Number 1, 2006. the peripheries. Cardiac Tissue-Restricted Deletion of Plakoglobin Results in Penn Vet | FAQs - University of Pennsylvania School of Veterinary Medicine Dr. Richa will maintain direct contact with investigators using the facility. Persing DH, McGinty L, Adams CW, Fowler RG. Department of 3800 Spruce Street - Philadelphia, PA 19104, Cas9 protein provided and final injection mixture done in house (unless otherwise specified), Injections done in mixed B6/B6D2 Background, but we also offer pure B6 Background upon request (injection into any other background will incur extra fees), Tail samples will be provided for genotyping by investigators, Desired pups will be transferred at weaning age (21 days), Chimeras will be determinable by coat color, Chimeras will be transferred at weaning age (21 days), The cost of your injection includes 2 clones from one target, We inject an average of 30 embryos per clone, Requesting lab must provided sperm sample, Can be performed using fresh or frozen sperm. DIR Core Facilities | NHLBI, NIH A primary function of the core is to provide a centralized laboratory that will generate infection-free strains of mice carrying transgenes or targeted alterations of specific interest to individual projects in the DRC. Pricing is discounted for bulk service. Steven's animal research career began at University of Pennsylvania as an Animal Laboratory Technician. Academic Services, Office of College Houses and. We are listed as VETTRAN. Basic Life Sci. To achieve these goals, the School of Veterinary Medicine has established the Transgenic Mouse Core. By testing other negative 2AP derivatives, the structural requirements necessary for AT----CG transversion mutagenesis have been determined. Jean Richa, Ph.D., is the Technical Director in charge of the production of transgenic mice. 2010 Feb; 89 (2): 165-169. doi: 10.1177/0022034509358392 PMCID: PMC2989847 NIHMSID: NIHMS199889 The Transgenic Mouse Core at the Center ( Technical director, N. Adrian Leu; Faculty oversight: P. Jeremy Wang MD/PhD and Christopher Lengner, PhD) provides an array of embryological services for the generation and preservation of genetically engineered transgenic and gene knockout mice. Transfer applicants must have completed a minimum of eight transferable college courses, including the following prerequisites: Introduction to Anatomy and Physiology 1 with Lab or Human Anatomy with Lab. Jean Richa, Ph.D., is the Technical Director in charge of the production of transgenic mice. Contact Us Transgenic Mouse Core Contacts Director: N. Adrian Leu Phone: 215-573-2302 Email : nleu@vet.upenn.edu Transgenic Services, Re-derivation Lab Manager: Stephanie Sterling Phone: 215-573-2301 Email: sterlin@vet.upenn.edu Cryopreservation, Billing, Shipping & Receiving Faculty Oversight: Dr. Jeremy Wang Email: pwang@vet.upenn.edu The School of Nursing is accredited by the Commission on Collegiate Nursing Education (CCNE). Estimated DEM uncertainty in creating a 3-D of the UPM's Ayer . TRANSGENIC ANIMALS are animals possessing a modified genome. 1985;31:381-9. doi: 10.1007/978-1-4613-2449-2_23. D. . . Mutagenicity of 2-aminopurine, 6-N-hydroxylaminopurine, and 2-amino-N6-hydroxyadenine in Neurospora crassa. PDF Biomedical Research Core Facilities - Perelman School of Medicine at The Molecular Pathology and Imaging Core is a resource for researchers at Penn, CHOP, and beyond. The last 4 analogues also cause transitions. 1993 Oct;101 Suppl 3(Suppl 3):21-6. doi: 10.1289/ehp.93101s321. Adele Harman: harmana@chop.edu: Translational Core. (J.Cell Biol. kpiotro@emory.edu. If freezing down current breeding males, please remove from breeding cage 1 week prior to freezing. and re-derivation of live and cryopreserved lines. Our liberal arts program helps prepare you for clinical, research, and leadership roles. If you have a bachelor's degree in a non-nursing discipline . This facility consists of an animal room and several injection rooms. Redirecting to /core/journals/journal-of-dairy-research/article/abs/expression-of-bovine-lactoglobulin-transgenic-mice Phone: 215-898-0198 | Fax 215-898-5408, Trustees of the University of Pennsylvania. PDF University of Pennsylvania Perelman School of Medicine High Throughput PDF Disposal of Transgenic Arthropods Compliance with the NIH - UPenn | Sitemap, Biomedical Research Core Facilities Newsletter, ABRF is a unique membership association comprising over. Dr. Karolina Nitsche is the EICF Scientist and Core Director for the Mouse Transgenic and Gene Targeting Core Facility since 2019. . Kozmin SG, Schaaper RM, Shcherbakova PV, Kulikov VN, Noskov VN, Guetsova ML, Alenin VV, Rogozin IB, Makarova KS, Pavlov YI. Genomic Analysis Core - Abramson Cancer Center | Penn Medicine Bethesda, MD 20894, Web Policies . MeSH Penn A-Z | University of Pennsylvania official website and that any information you provide is encrypted BUMC: Transgenic Mice Core . University of Pennsylvania Perelman School of Medicine Transgenic and RNA-seq reads were aligned to the . Klang-Langat Valley EMR is mainly concentrated in the core and internal areas of the region. The animal room provides housing and breeding space for the mice involved in the transgenic projects. Rm 720 BRB II/III Memo 3/18/15 regarding PSOM Subvention Requests for Mouse Cryopreservation Services. TRANSGENIC ANIMALS used at Penn include but are not limited to insects, nematodes, fish, amphibians, and rodents. Direct microinjection into the pronuclei of fertilized single-cell mouse embryos. A transgenic rescue of the AmelX / enamel using the full-length amelogenin gene (M180) was largely successful and restored enamel prism structure and properties (Fig. The purpose of the Transgenic & Chimeric Mouse Facility is to provide a centralized service to efficiently produce transgenic mice for basic research. BCM Mouse Genome Project - Large scale ENU based mouse mutagenesis for developmental defects; BUMC: Transgenic Mice Core - The mouse core facility at Boston University School of Medicine provides centralized, expert service to produce successfully transgenic as well as knock out mice for investigators at Boston University and as an outside service. Report Accessibility Issues and Get Help | Scientists at the School of Veterinary Medicine performed pioneering studies in the development of transgenic techniques and were responsible for production of the first transgenic mice and farm animals. Despite the advantage of adopting technologies, the elderly are slower to adopt new technologies compared The site is secure. The Transgenic and Chimeric Mouse Facility provides a centralized service to efficiently produce infection-free transgenic, chimeric, and genome-edited strains of mice. supported browser. Philadelphia, PA 19104, The Trustees of the University of Pennsylvania | Site best viewed in a Advancing Women in Engineering. Current studies at the Center are focused on exploring the biology of germline stem cells, as well as male and female gametes: Professor of Swine Health and Reproduction Bookshelf Fine-scale transgenic mapping of the MyoD core enhancer: MyoD is On increasing the hydroxylaminopurine concentration, the mutation frequency also increased concomitantly. We request that any scientific publications involving these transgenic animals acknowledge the services of the University of Pennsylvania . Perelman School of Medicine Moved Permanently. The facility uses sterile food and water as well as autoclaved cages and bedding; all cages are of the microisolator type to limit the spread of colony infection. Go to: Abstract Amelogenin (AMELX) and matrix metalloproteinase-20 (MMP20) are essential for proper enamel development. In laymens terms, they are siblings born to the same surrogate mother, exhibiting different combinations of the same genetic mixture. The mission of the core is to offer a "turn-key service" for new animal models at PBRC that will allow for the controlled manipulation of gene expression (knock-in point mutations, knock-in of cre recombinase, knock-in of floxed transcriptional blockers, and knock-in of human cDNA, into homologous mouse genes etc) and facilitate investigators. The Transgenic Mouse Genome Editing Core has generated a wide range of transgenic and gene-targeted mouse lines that have proven to be powerful tools for the study of the molecular biology and physiology of Type I and Type II diabetes, obesity, and developmental and mechanistic bases for a wide variety of other disorders of endocrinology and metabolism. The Core also oversees a cyropreservation facility for long-term storage of mouse embryos and sperm samples. The tryptophan synthetase gene A series of mutants in E. coli has been used to examine the mutational specificity of over 80 purine base analogues. An official website of the United States government. Penn Vet | Research Center - University of Pennsylvania School of Injection of a single sperm into cytoplasm of oocyte. When will I receive my transgenic mice? Techniques have been developed to enable the modification of individual genes in animals and plants and thereby precisely alter inherited traits. Mutat Res. Elucidation of epigenetic control of transposable elements in the germline and across generations. The injection rooms are fully equipped to carry out the entire procedure of making transgenic mice. <!-- /* Font Definitions */ @font-face {font-family:"MS Mincho"; panose-1:2 2 6 9 4 2 5 8 3 4; mso-font-alt:" "; mso-font-charset:128; mso-generic-font-family:modern; mso-font-pitch:fixed; mso-font-signature:-536870145 1791491579 134217746 0 131231 0;} @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;} @font-face {font-family:Times; panose-1:0 0 5 0 0 0 0 2 0 0; mso-font-alt:Times; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:-536870145 1342185562 0 0 415 0;} @font-face {font-family:Garamond; panose-1:2 2 4 4 3 3 1 1 8 3; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:647 2 0 0 159 0;} @font-face {font-family:Perpetua; panose-1:2 2 5 2 6 4 1 2 3 3; mso-font-charset:77; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:3 0 0 0 1 0;} @font-face {font-family:"\@MS Mincho"; panose-1:2 2 6 9 4 2 5 8 3 4; mso-font-charset:128; mso-generic-font-family:modern; mso-font-pitch:fixed; mso-font-signature:-536870145 1791491579 134217746 0 131231 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Garamond",serif; mso-fareast-font-family:"MS Mincho"; mso-fareast-theme-font:minor-fareast; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-family:"Garamond",serif; mso-ascii-font-family:Garamond; mso-fareast-font-family:"MS Mincho"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Garamond; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}size:8.5in 11.0in; margin:1.0in 1.0in 1.0in 1.0in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.WordSection1 {page:WordSection1;}. HHS Vulnerability Disclosure, Help Core also provides embryo and sperm cryopreservation as well as in vitro fertilization and re-derivation of live and cryopreserved lines, along with long-term . Amelx and Mmp20 mutations cause amelogenesis imperfecta. Transgenic Core. These mice carry randomly inserted transgenes and/or site-specific alterations in the mouse genome of specific interest to Penn researchers. 2A6MAP, 2-amino-6-ethylaminopurine and 2,6-bismethylaminopurine stimulate only the AT----CG transversion while 2MAP additionally gives rise to AT----TA transversions. 1978 Jan;56(3):225-34. doi: 10.1016/0027-5107(78)90189-6. de Serres FJ, Brockman HE, Hung CY, Overton LK. Transgenic and Chimeric Mouse Facility . A variety of clinical experiences offer a real-world view, so you can discover whats right for you. University of Pennsylvania Study of molecular mechanisms of mammalian sperm motility and testicular degeneration has implications for treatment of infertility.